Cyp19a1b-gfp
WebMay 7, 2012 · The tg(cyp19a1b-GFP) transgenic zebrafish expresses GFP (green fluorescent protein) under the control of the cyp19a1b gene, encoding brain aromatase. …
Cyp19a1b-gfp
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WebNov 30, 2011 · We then characterized the neuronal or radial glial nature of the Pgr expressing cells by performing double staining with acetylated-tubulin (neuronal marker) or by using the tg ( cyp19a1b -GFP) transgenic zebrafish, which specifically expresses GFP in radial glial cells [38], [49], [51]. WebFeb 11, 2024 · In addition, gene knockout of all three isoforms of esrs or gonad-specific aromatase ( cyp19a1a) in zebrafish embryos collectively confirm their roles in differentiation and development of female reproductive organs (i.e., defects in follicle development in the ovary) ( Lau et al., 2016; Lu et al., 2024 ), similar to that for ERα - and aromatase …
WebMar 18, 2024 · Title: A cyp19a1b-gfp (aromatase B) transgenic zebrafish line that expresses GFP in radial glial cells. Cyp19b is regulated by estrogens.Promoter … Web开馆时间:周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆
WebMar 11, 2024 · Newly fertilized cyp19a1b-GFP eleutheroembryos were transferred to glass crystallizers and exposed from 0- to 4-dpf in an incubator (28 ± 1°C) under semi-static conditions with a total renewal of the medium each day. Each condition consisted in a triplicate of crystallizers containing 7 embryos and 15 ml of water. Webcyp19a1a. Predicted to enable several functions, including heme binding activity; iron ion binding activity; and monooxygenase activity. Acts upstream of with a positive effect on …
WebSep 15, 2016 · The cyp19a1b -GFP transgenic zebrafish ( Tong et al., 2009) were held at the Institut National de l'Environnement Industriel et des Risques (INERIS, Verneuil-en-Halatte, France). They were maintained in 3.5 L aquaria in a recirculation system (Zebtec, Tecniplast, Buguggiate, Italy) on a 14 h light: 10 h dark cycle at a temperature of 25.1 ± …
WebA cyp19a1b-GFP (aromatase B) transgenic zebrafish line that expresses GFP in radial glial cells Sok Keng Tong, Karen Mouriec, Ming Wei Kuo, Elisabeth Pellegrini, Marie Madeleine Gueguen, François Brion, Olivier Kah, Bon Chu Chung Faculty of Medical Sciences Research output: Contribution to journal› Article› peer-review 103Citations (Scopus) how much are tortoises at petcoWebAug 15, 2013 · The tg(cyp19a1b-GFP) zebrafish strain is stably transfected with a green fluorescent protein (GFP) gene that is regulated by the zebrafish cyp19a1b (brain … how much are train tickets to nycWebThe EASZY assay is a mechanism-based in vivo screening assay designed to detect endocrine active chemicals acting as agonist through estrogen receptors (ERs), by inducing the expression of the green fluorescent protein (GFP) driven by the cyp19a1b promoter. The EASZY assay allows for the detection of estrogenic activity of chemicals on … how much are toyota supra mk4WebFurthermore, E2 treatment of cyp19a1b-GFP (green fluorescent protein) transgenic embryos results in appearance of GFP expression in the brain as early as 25 hpf. These … how much are tracfone cardsWebcyp19a1b-GFPzebrafish line, we de- termined thatcypa1bup-regulation by E2 is detectable as early as 25 hpf. Altogether these data indicate that thecyp19a1bgene is a target for es- trogens and xenoestrogens at very early stages of development. RESULTS Early Expression of esr1, esr2b, and esr2a mRNAs how much are trailersWebDec 19, 2008 · To understand the function of AroB in the brain, we generated transgenic zebrafish that expresses green fluorescent protein (GFP) driven by the brain aromatase cyp19a1b promoter. GFP was found in the radial glial cells of transgenic larvae and adult fish that overlap with AroB immunoreactivity in the correct temporal and spatial pattern. photoreflect software downloadWebNov 20, 2024 · The transgene can be integrated into the host genome by several methods, which are the most common: Transposons, mediated by nucleases such as Zinc Fingers nucleases (ZFNs), Transcription activator-like effector nucleases (TALENs), and RNA-guided nucleases such as endonuclease CRISPR/Cas9 (clustered regularly interspersed … how much are toyota