Ctd110.6 antibody

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August undefined, 2024

WebO-GlcNAc Antibody (CTD110.6) is an IgM κ mouse monoclonal O-GlcNAc antibody (also designated O-linked β-N-acetylglucosamine antibody, O-β-GlcNAc antibody, O-linked … WebThus, with careful controls, antibodies CTD110.6 (IgM), #3 (IgG), and #10 (IgG) can be used to detect membrane and secreted proteins modified by O-GlcNAc on EGF repeats. Secreted and cell surface glycoproteins carry a wide spec-trum of N-and O-glycans that are synthesized during transit

Significant Roles of Notch O-Glycosylation in Cancer

Web开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 WebImmunofluorescence staining of CTD110.6 on methanol fixed HeLa cells at 10 µg/ml. Following fixation, the cells were incubated with the primary antibody for one hour at … beatelmania

Alexa Fluor 594 anti-O-GlcNAc Antibody anti-O-GlcNAc - CTD110.6 …

WebThe antibody CTD110.6 is often used to detect changes in the O-GlcNAc modification. Recently, it has been demonstrated that CTD110.6 recognizes N-linked N,N'-diacetylchitobiose, which is thought ... WebApr 18, 2014 · Binding by antibodies CTD110.6, #3, and #10 was inhibited by 5 m m GlcNAc, but not 5 m m GalNAc, and was thus specific for recognition of O-GlcNAc. However, binding of antibodies 10D8, RL2, and #14 was not inhibited by the presence of 5 m m GlcNAc. Therefore, only antibodies CTD110.6, #3, and #10 should be used for cell … WebMar 5, 2024 · Compared to immunostaining of O-GlcNAcylated proteins by CTD110.6 antibody, OGT immunostaining appears to be more cytosolic with less intense immunoreactivity in hippocampal CA1 (ratios of immunoreactivity intensity between CA1 to adjacent region are ~ 0.73 for OGT and ~ 2.5 for CTD110.6, respectively) or dentate … digestive naranja

TotalSeq™-C0106 anti-mouse CD11c Antibody - BioLegend

O-GlcNAc (CTD110.6) Mouse mAb (HRP Conjugate) 12938S from …

WebApr 18, 2014 · We show that CTD110.6, #3, and #10 antibodies can be used to detect cell surface glycoproteins bearing O-GlcNAc. Cell surface glycoproteins recognized by … WebIn 2008, the Okajima group originally discovered the O-GlcNAc modification on Drosophila Notch EGF20 by detecting O-glycans on the ECD of Drosophila Notch using an antibody that recognizes O-GlcNAc (CTD110.6) . Later, O-GlcNAc modifications of the EGF repeats in Drosophila Delta, Serrate, and Dumpy were subsequently discovered [86,87]. digestivna hirurgijaWebClone CTD110.6 specifically detected serine and threonine residues with beta-O-linked GlcNAc, but not alpha-O-linked GlcNAc. The presence of GlcNAc, but not GalNAc, abolished the detection of the target modification. Clone CTD110.6 did not recognize peptides with unmodified serine and threonine residues (Comer, F.I., et al. (2001). beaten conjugaison

"WebThe β-O-linked N-acetylglucosamine (O-GlcNAc) antibody recognizes glycosylation, a post-translational modification, which occurs on serine and threonine residues of proteins in. Ximbio. Find reagents. ... Anti-O-GlcNAc (CTD110.6) antibody Invented at ... " - Ctd110.6 antibody

Ctd110.6 antibody

O-GlcNAc monoclonal antibody (CTD110.6) (purified)

WebSimilar results were obtained for wild-type HEK293T cells (not shown). (C) Expression and O-GlcNAcylation of Notch1, Notch1Δ2xO-GlcNAc and Notch1Δ4xO-GlcNAc were analyzed by immunoprecipitation (IP) using NOTCH1 (8G10) antibody, followed by immunoblotting with CTD110.6 O-GlcNAc or NOTCH1 antibodies. WebAntibody: Clone: CTD110.6: Immunogen : This antibody was raised against a peptide containing O-GlcNAc - serine. This antibody recognizes single O-GlcNAc residues in b …

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WebJan 13, 2003 · Anti-CD46 antibodies inhibit binding of Ad7 and Ad11 virions to CHO-BC1 cells. In a second experiment, we preincubated A549 cells or CHO-BC1 cells with … WebBinding by antibodies CTD110.6, #3, and #10 was inhibited by 5 m m GlcNAc, but not 5 m m GalNAc, and was thus specific for recognition of O-GlcNAc. However, binding of antibodies 10D8, RL2, and #14 was not inhibited by the presence of 5 m m GlcNAc. Therefore, only antibodies CTD110.6, #3, and #10 should be used for cell surface …

WebO-GlcNAc (CTD110.6) is a mouse monoclonal antibody raised against a peptide containing serine-O-linked N-acetylglucosamine. PRODUCT Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of Webat 6 h after the treatment, i.e. at the same time that an increase was previously noted for proteins identified by their reaction with CTD110.6 antibodies (Figure 1b). The immunoprecipitation analysis showed that CTD110.6 antibodies reacted with all four of the lower molecular weight forms that were immunoprecipitat-

WebO-GlcNAc monoclonal antibody (CTD110.6) (purified) O-GlcNAc单克隆抗体（CTD110.6）（纯化的） ... VEGF Recombinant monoclonal antibody (A.4.6.1) (Mouse IgG1κ) 血管内皮生长因子重组单克隆抗体（A.4.6.1）（小鼠IgG1κ） ... WebFigure 6 HRECs exposed to TNF-alpha, and high glucose for 24 h demonstrated alterations in VE-Cadherin and defective complex formation. The whole cell lysates were immunoprecipitated with indicated antibodies, and the immunocomplexes were analyzed for O-GlcNAcylation using anti-O-GlcNAc antibody (clone CTD110.6) ( A ).

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WebCTD110.6 : Host: Mouse : Isotype: IgM : Immunogen: Synthetic peptide containing serine-O-GlcNAc. Species reactivity: Species independent : Specificity: Recognizes Ser-O-GlcNAc … digestive gdzie kupićWebDec 11, 2009 · The following antibodies were used: anti-O-GlcNAc (CTD110.6, Covance), anti-OGT (DM17, Sigma), anti-actin, anti-glycogen phosphorylase ... Korea) for providing the glutamine:fructose-6-phosphate amidotransferase antibody. Supplementary Material. Download : Download Acrobat PDF file (541KB) Recommended articles. REFERENCES … digestive juice nameWebMay 18, 2024 · 3. Incubate the blots with CTD110.6 (diluted to 1 μg/ml in antibody dilution buffer), in duplicate, with and without 100 mM GlcNAc, overnight at 4°C. An antibody that has been antigen purified using a GlcNAc-agarose column yields higher-quality blots. Commercially available CTD110.6 is purified by ammonium sulfate precipitation. digestivo asisa jerezWebPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. digestive koekjes jumboWebO-GlcNAc antibody (mAb) (Clone CTD110.6) for use in Immunoprecipitation (IP) ... This antibody recognizes single O-GlcNAc residues in b-O-glycosidic linkage to serine and … beaten bap sandwichhttp://bb-china.net/search.html?brand=42&pcategory=0&classd=0&use=0&kw=&promotions=2&inventory=2&category=17&page=21 digestive koekjes glutenvrijWebFor O-glcNAcylation detection, CTD110.6 antibody was covalently conjugated to (4) Defective STIM-mediated store operated Ca2+ entry in hepatocytes leads to metabolic dysfunction in obesity eLife December 15, 2024 Ana Paula Arruda, Benedicte Mengel Pers, Günes Parlakgul, Ekin Güney, Ted Goh, ... beaten badly