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Web100 x 50 µl reactions, real-time PCR mix, 2x mix contains dNTPs, 50 U/ml iTaq™ DNA polymerase, 6 mM MgCl 2, SYBR ® Green I, 20 nM fluorescein. iQ™ SYBR® Green Supermix, 100 x 50 µl rxns, 2.5 ml (2 x 1.25 ml) Buy Now. Place your order directly with the manufacturer. Reviews. WebUse iTaq Universal SYBR Green Supermix with blended ROX and fluorescein passive reference dyes for dye-based quantitative PCR (qPCR).Ready-to-use mix optimized for dye-based qPCR on any real-time PCR instrument.ontains both ROX and fluorescein dyes for universal real-time instrument compatibility (ROX-independent and ROX-dependent … florida couple and dog chased by bear

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WebMar 3, 2024 · Quantitative PCR was performed in 20 μL reactions with 200 nM of each primer, iTaq Universal SYBR Green Supermix (Biorad, Bio-Rad, Des Plaines, IL, USA) and 1 μL of cDNA template using the QuantStudio 3 Real-time PCR system (Applied Biosystems, Waltham, MA, USA). Primers used were: GNMT forward 5′ … WebFeb 28, 2014 · In general, chemistries consist of special fluorescent dye and probes in the PCR including DNA-binding dyes such as EtBr or SYBR Green I, hydrolysis probes, hybridization probes, molecular beacons and peptide nucleic acid light-up, sunrise and scorpion primers probes.[1,2,3,4] SYBR Green I when binds to the minor groove of … WebiTaq™ Universal SYBR® Green One-Step Kit, 100 x 20 µl rxns, 1 ml by Bio-Rad. 1 ml, one-step RT-PCR kit, includes 2x one-step SYBR Green reaction mix (Taq polymerase, … florida county veteran service officers